DNA Vectors
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ANTIBODY DESIGN LABS PADL-100 IPHAGEMID VECTOR
pADL™-100 is a phagemid vector designed for high levels of display on the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence followed by a full-length copy of the gene III sequence and a double-SfiI cloning site to introduce foreign polypeptides on the N-terminal side of the gene III protein. Expression of the fusion is under the tight control of a lac promoter. The presence of a strong RBS, a proteolysis-resistant linker without tags and the absence of amber stop codon all contribute to maximize display on pIII.
